5CNR

Crystal structure-guided design of self-assembling RNA nano triangles

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-01-13
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	H 3 2
Unit cell lengths	59.934, 59.934, 262.622
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.403 - 2.590
R-factor	0.1979
Rwork	0.193
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p97
RMSD bond length	0.002
RMSD bond angle	0.445
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.680
High resolution limit [Å]	2.590	2.590
Number of reflections	5011
<I/σ(I)>	35.5
Completeness [%]	83.2	11.7
Redundancy	17.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	281	drop: 20% MPD, 55 mM NaCl, 12 mM spermine, 40 mM Tris well: 18% MPD, 120 mM NaCl