5CNQ

Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA, Mg2+ and Mn2+ ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.91376
Spacegroup name	P 31 2 1
Unit cell lengths	98.582, 98.582, 119.619
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.291 - 2.602
R-factor	0.214
Rwork	0.212
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5co8
RMSD bond length	0.005
RMSD bond angle	0.874
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX (1.9-1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.291	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.131	0.567
Number of reflections	21142
<I/σ(I)>	6.5	1.9
Completeness [%]	99.9	99.4
Redundancy	19.8	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	279	PEG10000, NaCl, magnesium chloride