5CNK
mglur3 with glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-05 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97929 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.344, 80.004, 206.861 |
Unit cell angles | 90.00, 130.73, 90.00 |
Refinement procedure
Resolution | 78.380 - 3.150 |
R-factor | 0.2222 |
Rwork | 0.221 |
R-free | 0.25800 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 78.380 |
High resolution limit [Å] | 3.150 |
Number of reflections | 29288 |
<I/σ(I)> | 3.5 |
Completeness [%] | 97.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 16% PEG 3350 + 200mM Ammonium Iodide, 50mM Tris pH 8.0 / 150mM NaCl |