5CN2
Crystal structure of yeast GGA1_GAE domain-C2221
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.040, 110.940, 90.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.630 - 2.250 |
| R-factor | 0.23317 |
| Rwork | 0.231 |
| R-free | 0.27016 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cn1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.546 |
| Data reduction software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 13301 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 99.8 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 289 | 20% PEG 5000 MME, 0.2 M (NH4)2SO4, 0.1M Tris pH 8.5 |
