5CN1
Crystal structure of yeast GGA1_GAE domain-P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.007, 53.585, 71.547 |
| Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
| Resolution | 42.480 - 2.310 |
| R-factor | 0.21519 |
| Rwork | 0.212 |
| R-free | 0.26972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mnm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.627 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 21922 |
| <I/σ(I)> | 15.3 |
| Completeness [%] | 99.6 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 289 | 1.2 M Potassium sodium tartrate tetrahydrate, 0.1M Tris pH 8.5 |
