5CMZ
Artificial HIV fusion inhibitor AP3 fused to the C-terminus of gp41 NHR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 63 |
| Unit cell lengths | 44.403, 44.403, 227.897 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.983 - 2.574 |
| R-factor | 0.2458 |
| Rwork | 0.245 |
| R-free | 0.26420 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.471 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.000 |
| High resolution limit [Å] | 2.570 |
| Number of reflections | 61523 |
| <I/σ(I)> | 27.2 |
| Completeness [%] | 99.9 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2M Ammonium Sulfate, 0.1M Bis-Tris pH 6.5, 25% w/v PEG 3350 |
