5CMW
Crystal structure of yeast Ent5 N-terminal domain-soaked in KI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.5498 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.290, 51.760, 59.080 |
| Unit cell angles | 90.00, 103.42, 90.00 |
Refinement procedure
| Resolution | 38.490 - 2.200 |
| R-factor | 0.20874 |
| Rwork | 0.205 |
| R-free | 0.25810 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.287 |
| Data reduction software | MOSFLM |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 8783 |
| <I/σ(I)> | 11.9 |
| Completeness [%] | 99.4 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 289 | 0.5 M Potassium thiocyanate, 0.1M BIS-TRIS propane pH 7.0 |
