5CMC
Mnemiopsis leidyi ML032222a iGluR LBD E423S mutant glycine complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.899, 123.138, 54.507 |
Unit cell angles | 90.00, 111.72, 90.00 |
Refinement procedure
Resolution | 39.109 - 1.280 |
R-factor | 0.1355 |
Rwork | 0.134 |
R-free | 0.15680 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4yki |
RMSD bond length | 0.027 |
RMSD bond angle | 1.267 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1760) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.300 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.040 | 0.323 |
Number of reflections | 144111 | |
<I/σ(I)> | 34.92 | 3.5 |
Completeness [%] | 96.6 | 81.9 |
Redundancy | 4.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | Reservoir: 24% PEG 8K, 100 mM Cacodylate, 150 mM MgS04. Protein buffer: 150 mM NaCl, 10 mM HEPES, pH 7.0, 0.5 mM EDTA, 25 mM glycine |