5CMB
Mnemiopsis leidyi ML032222a iGluR LBD R703K mutant glycine complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.731, 123.389, 54.334 |
| Unit cell angles | 90.00, 112.20, 90.00 |
Refinement procedure
| Resolution | 38.988 - 1.340 |
| R-factor | 0.1444 |
| Rwork | 0.143 |
| R-free | 0.16670 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4yki |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.239 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.360 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.045 | 0.365 |
| Number of reflections | 124741 | |
| <I/σ(I)> | 31.1 | 2.4 |
| Completeness [%] | 97.8 | 72.9 |
| Redundancy | 4.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | Reservoir: 24% PEG 8K, 100 mM Cacodylate, 150 mM MgS04. Protein buffer: 150 mM NaCl, 10 mM HEPES, pH 7.0, 0.5 mM EDTA, 10 mM glycine |
