5CM4
Crystal structure of human Frizzled 4 Cysteine-Rich Domain (CRD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.078 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 105.583, 111.738, 75.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.2274 |
Rwork | 0.226 |
R-free | 0.25521 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ijy |
RMSD bond length | 0.010 |
RMSD bond angle | 1.819 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.072 | 0.990 |
Number of reflections | 17684 | |
<I/σ(I)> | 17.6 | 2 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 6.2 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 1.8 M ammonium sulfate, 0.1 M tri-Na citrate pH 5.6 and 0.2 M Na/K tartrate |