5CM3
Crystal Structure of KorA, a plasmid-encoded, global transcription regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.540, 115.100, 49.930 |
| Unit cell angles | 90.00, 113.81, 90.00 |
Refinement procedure
| Resolution | 23.459 - 2.302 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ckt theoretical model of DNA |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.482 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.460 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.032 | |
| Number of reflections | 19920 | |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 97.6 | 90.1 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | PEG 6000, sodium acetate, ethylene glycol |
