5CM2
Structure of Y. lipolytica Trm9-Trm112 complex, a methyltransferase modifying U34 in the anticodon loop of some tRNAs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9801 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 176.200, 176.200, 176.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.050 - 2.500 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.24200 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.193 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.650 |
High resolution limit [Å] | 2.494 | 2.500 |
Rmerge | 0.501 | |
Number of reflections | 16239 | |
<I/σ(I)> | 14.2 | 2.5 |
Completeness [%] | 97.9 | 98.3 |
Redundancy | 4.85 | 4.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 100MM TRI-SODIUM CITRATE, 20% POLYETHYLENE GLYCOL 4000 (PEG4K), 20% 2-PROPANOL, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 100MM TRI-SODIUM CITRATE, 20% POLYETHYLENE GLYCOL 4000 (PEG4K), 20% 2-PROPANOL, PH 7.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K |