5CLV
Crystal Structure of KorA-operator DNA complex (KorA-OA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.460, 114.030, 82.070 |
| Unit cell angles | 90.00, 99.59, 90.00 |
Refinement procedure
| Resolution | 25.047 - 2.500 |
| R-factor | 0.2798 |
| Rwork | 0.279 |
| R-free | 0.29240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ckt theoretical DNA model |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.797 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.050 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.038 | 0.352 |
| Number of reflections | 49516 | |
| <I/σ(I)> | 12.4 | 2.8 |
| Completeness [%] | 97.9 | 99.8 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | PEG 4000, sodium acetate, ethylene glycol |
