5CLR
Crystal structure of LegK4_APO Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2013-11-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 154.820, 154.820, 87.955 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.693 - 3.706 |
| R-factor | 0.2835 |
| Rwork | 0.282 |
| R-free | 0.31070 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.538 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.693 | 3.838 |
| High resolution limit [Å] | 3.700 | 3.706 |
| Rmerge | 0.239 | 1.419 |
| Number of reflections | 13215 | |
| <I/σ(I)> | 13.79 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 2.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12% (w/v) PEG 4000, 20% glycerol, 0.1 M MOPS/HEPES pH 7.5, 5 mM Hexaammine cobalt (III) chloride. |
