5CL2

Crystal structure of Spo0M, sporulation control protein, from Bacillus subtilis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-11
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.90
Spacegroup name	P 21 21 21
Unit cell lengths	65.995, 74.228, 133.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.620 - 2.300
R-factor	0.2297
Rwork	0.227
R-free	0.27270
RMSD bond length	0.010
RMSD bond angle	1.387
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.056	0.510
Number of reflections	29843
<I/σ(I)>	48.99	4.9
Completeness [%]	99.6	96.8
Redundancy	7.6	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M Tris-HCl, pH 7.0, 1.0-1.1 M Na-citrate