5CKY
Crystal Structure of the MTERF1 R162A substitution bound to the termination sequence.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.217, 91.161, 159.103 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.550 - 2.620 |
R-factor | 0.2164 |
Rwork | 0.214 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mva |
RMSD bond length | 0.012 |
RMSD bond angle | 1.689 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.550 | 2.624 |
High resolution limit [Å] | 2.615 | 2.615 |
Rmerge | 0.066 | 0.807 |
Number of reflections | 19776 | |
<I/σ(I)> | 25.4 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Sodium Acetate, 0.1M Tris HCl, 15.5% PEG4000 |