5CKM
The CUB1-EGF-CUB2 domains of rat MBL-associated serine protease-2 (MASP-2) bound to Ca2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-08 |
Detector | RIGAKU |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.900, 119.390, 104.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.900 - 2.730 |
R-factor | 0.2069 |
Rwork | 0.204 |
R-free | 0.26560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nto |
RMSD bond length | 0.004 |
RMSD bond angle | 0.830 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.900 | 2.820 |
High resolution limit [Å] | 2.730 | 2.730 |
Number of reflections | 14031 | |
<I/σ(I)> | 6.4 | 2.06 |
Completeness [%] | 99.4 | 94.4 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris-Ac pH 8 containing 40% MPD and 200 mM sodium citrate |