5CKI
Crystal structure of 9DB1* deoxyribozyme (Cobalt hexammine soaked crystals)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.604 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 80.845, 80.845, 55.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.930 - 2.985 |
R-factor | 0.2591 |
Rwork | 0.256 |
R-free | 0.31090 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.599 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX |
Refinement software | PHENIX (1.10pre_2086) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.930 | 3.090 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.080 | 3.990 |
Number of reflections | 4060 | |
<I/σ(I)> | 25.23 | 0.86 |
Completeness [%] | 100.0 | 98 |
Redundancy | 4 | 24 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | MPD, spermine, magnesium chloride, sodium chloride, sodium cacodylate |