5CKH
E. coli MazF E24A form IIb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.199, 64.199, 224.535 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.631 - 2.449 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.083 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.630 | 2.540 |
| High resolution limit [Å] | 2.449 | 2.449 |
| Rmerge | 0.590 | |
| Number of reflections | 10883 | |
| <I/σ(I)> | 20.54 | 4.4 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 10.1 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.1M MOPS/Na Hepes pH 7.5, 0.02M of each a.a.,12.5% PEG1000, 12,.5% PEG3350, 12.5% MPD |
