5CKF
E. coli MazF E24A form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0723 |
| Spacegroup name | P 61 |
| Unit cell lengths | 52.816, 52.816, 191.398 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.630 - 2.800 |
| R-factor | 0.2387 |
| Rwork | 0.236 |
| R-free | 0.29980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub4 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.630 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 8844 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 97.5 | 96.5 |
| Redundancy | 6.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.1M Disodium Citrate pH 7.5, 20% 2-propanol, 40% PEG400 |
