5CKE
E.coli MazF E24A form IIa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.8081 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 62.654, 62.654, 217.741 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.327 - 2.311 |
| R-factor | 0.2155 |
| Rwork | 0.212 |
| R-free | 0.27420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub4 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.965 |
| Data reduction software | XDS |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 11801 | |
| <I/σ(I)> | 12.9 | 2.9 |
| Completeness [%] | 96.8 | 97.6 |
| Redundancy | 7.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 292 | 0.1 MES pH 6.2, 1.6M Ammonium Sulfate, 10% (v/v) 1,4-dioxane |
