5CK9
E. coli MazF form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 61 |
| Unit cell lengths | 52.190, 52.190, 197.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.110 - 1.898 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.110 | 2.010 |
| High resolution limit [Å] | 1.898 | 1.898 |
| Number of reflections | 23960 | |
| <I/σ(I)> | 17.68 | 1.64 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 11.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.1M Disodium Citrate pH 5.6, 0.2M Ammonium Acetate, 30%MPD |
