5CK7
Mouse ADP-dependent Glucokinase; AMP bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2014-04-09 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.541800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.681, 58.492, 161.708 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.640 - 2.990 |
R-factor | 0.22817 |
Rwork | 0.227 |
R-free | 0.24315 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ccf |
RMSD bond length | 0.015 |
RMSD bond angle | 1.696 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.640 | 3.100 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.091 | 0.290 |
Number of reflections | 9031 | |
<I/σ(I)> | 10.1 | 2.6 |
Completeness [%] | 97.1 | 80.2 |
Redundancy | 5.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 294 | Seeding of preequilibrated drops of 0.2 M NH4Cl, 20% w/v PEG 3350 |