5CK4

Signal recognition particle receptor SRb-GDP from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.99
Spacegroup name	P 1
Unit cell lengths	34.960, 64.310, 64.930
Unit cell angles	86.46, 90.13, 79.58

Refinement procedure

Resolution	34.380 - 1.890
R-factor	0.1671
Rwork	0.165
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.046
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.380
High resolution limit [Å]	1.890
Rmerge	0.062
Number of reflections	44352
<I/σ(I)>	9.4
Completeness [%]	97.7
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291.15	0.2 M sodium acetate, 20% (w/v) PEG3350