5CK1
selenomethionine BT4246
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.792, 115.738, 116.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.827 - 1.835 |
| R-factor | 0.1682 |
| Rwork | 0.167 |
| R-free | 0.20130 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.925 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.830 | 1.901 |
| High resolution limit [Å] | 1.835 | 1.835 |
| Rmerge | 0.110 | 0.213 |
| Number of reflections | 65430 | |
| <I/σ(I)> | 37.41 | 13.3 |
| Completeness [%] | 99.7 | 98.08 |
| Redundancy | 6.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG3350, KCl, nucleation off of a grain of SiO2. |
