5CJZ
BT4246 with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-10-04 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 155.925, 155.925, 114.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.880 - 1.803 |
| R-factor | 0.1762 |
| Rwork | 0.175 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ck1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.880 | 1.867 |
| High resolution limit [Å] | 1.803 | 1.803 |
| Rmerge | 0.230 | 1.797 |
| Number of reflections | 74235 | |
| <I/σ(I)> | 10.95 | 1.03 |
| Completeness [%] | 98.3 | 98.13 |
| Redundancy | 13.1 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, KCl |
