5CJZ
BT4246 with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-10-04 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.979 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 155.925, 155.925, 114.315 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.880 - 1.803 |
R-factor | 0.1762 |
Rwork | 0.175 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ck1 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.924 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.880 | 1.867 |
High resolution limit [Å] | 1.803 | 1.803 |
Rmerge | 0.230 | 1.797 |
Number of reflections | 74235 | |
<I/σ(I)> | 10.95 | 1.03 |
Completeness [%] | 98.3 | 98.13 |
Redundancy | 13.1 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, KCl |