5CJJ
The crystal structure of phosphoribosylglycinamide formyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924, 0.97937 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 65.162, 65.162, 368.905 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.420 |
| R-factor | 0.2515 |
| Rwork | 0.249 |
| R-free | 0.28960 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.666 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.110 | 0.662 |
| Number of reflections | 19055 | |
| <I/σ(I)> | 28.4 | 3.2 |
| Completeness [%] | 99.8 | 97.9 |
| Redundancy | 15.2 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M HEPES, 30% v/v Jeffamine ED-2001Reagent |
