5CJ9
Bacillus halodurans Arginine repressor, ArgR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 104.676, 104.676, 104.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.976 - 2.409 |
| R-factor | 0.1849 |
| Rwork | 0.182 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b4a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.240 |
| Data scaling software | MOSFLM (0.1.26) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.077 | 0.379 |
| Number of reflections | 8128 | |
| <I/σ(I)> | 44.3 | 12.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 39.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 296 | 5%(v/v) 1,2-propanediol, 0.1 M bicine pH 8.0 |
