5CJ4
Crystal Structure of Amino Acids 1562-1622 of MYH7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.145, 57.285, 112.100 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 49.923 - 3.102 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.27960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ik9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.741 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.070 | 0.366 |
| Number of reflections | 19051 | |
| <I/σ(I)> | 27.2 | 4.9 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 23% (w/v) PEG 4000, 500 mM NaCl, 100 mM triethanolamine pH 8.0. |
