5CIX
Structure of the complex of type 1 Ribosome inactivating protein with triethanolamine at 1.88 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 |
| Unit cell lengths | 130.262, 130.262, 38.158 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.570 - 1.880 |
| R-factor | 0.1686 |
| Rwork | 0.166 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jtp |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.794 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | Coot |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.570 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 19516 | |
| <I/σ(I)> | 0.0185 | 0.335 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG 6000, 0.1M Sodium Phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
