5CIU
Structural basis of the recognition of H3K36me3 by DNMT3B PWWP domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 73.437, 73.437, 158.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.718 - 2.240 |
R-factor | 0.2268 |
Rwork | 0.224 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qkj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.204 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.2) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 | |
High resolution limit [Å] | 2.240 | 6.610 | 2.240 |
Rmerge | 0.062 | 0.032 | 0.633 |
Rmeas | 0.067 | 0.035 | 0.683 |
Total number of observations | 168485 | ||
Number of reflections | 24436 | 998 | 3816 |
<I/σ(I)> | 18.44 | 37.58 | 2.95 |
Completeness [%] | 99.5 | 94.1 | 98.6 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 1.6M SODIUM CITRATE TRIBASIC |