5CIS
The CUB1-EGF-CUB2 domains of rat MBL-associated serine protease-2 (MASP-2) bound to Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.930, 98.300, 121.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.343 - 2.580 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nto |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.300 | 2.600 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.645 | |
| Number of reflections | 12626 | |
| <I/σ(I)> | 11.58 | 2.03 |
| Completeness [%] | 97.6 | 93.5 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris pH 8.5 containing 80 mM ammonium acetate |
