5CIQ
Ran GDP wild type tetragonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-03-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 41 |
| Unit cell lengths | 102.680, 102.680, 41.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.230 - 1.650 |
| R-factor | 0.19844 |
| Rwork | 0.196 |
| R-free | 0.24938 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human Ran GDP |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.257 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.230 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.050 | 0.059 |
| Number of reflections | 52073 | |
| <I/σ(I)> | 18.11 | 2.05 |
| Completeness [%] | 98.2 | 85.7 |
| Redundancy | 4.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 30% PEG 1000, 50 mM TRIS pH 8, 20 mM MgCl2 |
