5CIH
Complex of yeast cytochrome c peroxidase (W191Y) with iso-1 cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.451, 114.219, 88.258 |
| Unit cell angles | 90.00, 104.32, 90.00 |
Refinement procedure
| Resolution | 35.359 - 2.600 |
| R-factor | 0.2289 |
| Rwork | 0.223 |
| R-free | 0.30960 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.713 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.370 |
| High resolution limit [Å] | 2.330 | 6.320 | 2.330 |
| Rmerge | 0.135 | 0.060 | 0.566 |
| Rmeas | 0.157 | 0.071 | 0.750 |
| Rpim | 0.088 | 0.037 | 0.487 |
| Total number of observations | 88500 | ||
| Number of reflections | 28703 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 76.2 | 99.2 | 13 |
| Redundancy | 3.1 | 3.6 | 1.8 |
| CC(1/2) | 0.995 | 0.287 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350 15-15%, 100 mM NaAcetate, 175 mM NaCl |
