5CI6
Crystal structure of Arabidopsis thaliana MPK6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 150.513, 150.513, 85.624 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.700 - 3.000 |
| R-factor | 0.22823 |
| Rwork | 0.226 |
| R-free | 0.27234 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ERK |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.072 | 0.684 |
| Number of reflections | 24998 | |
| <I/σ(I)> | 16.48 | 2.15 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 295 | 0.1 M citrate, 10-12% PEG 20000 |
