5CHT
Crystal structure of USP18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.959, 89.747, 149.406 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.772 - 2.800 |
R-factor | 0.2279 |
Rwork | 0.225 |
R-free | 0.28290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nb8 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.059 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.301 | 2.771 |
Number of reflections | 18487 | |
<I/σ(I)> | 6.29 | 0.79 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 4.86 | 4.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.09 M succinate, pH 7.0, 13.5% (w/v) PEG 3350, 10 mM KBr |