5CH9
Gkap mutant B12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.822, 79.643, 90.066 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18504 |
| Rwork | 0.183 |
| R-free | 0.21268 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.392 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 55188 |
| <I/σ(I)> | 4.7 |
| Completeness [%] | 98.8 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 287 | PEG 3350 Tacsimate sodium citrate tribasic dihydrate |
