5CFF
Crystal structure of Miranda/Staufen dsRBD5 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 200.023, 51.332, 100.274 |
Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
Resolution | 27.664 - 2.500 |
R-factor | 0.2443 |
Rwork | 0.242 |
R-free | 0.28100 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.183 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
Rmerge | 0.078 | 0.045 | 0.664 |
Total number of observations | 189671 | ||
Number of reflections | 34191 | ||
<I/σ(I)> | 13.9 | ||
Completeness [%] | 94.9 | 88.1 | 99.8 |
Redundancy | 5.5 | 4.8 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 289 | sodium acetate,1,6-hexanediol,calcium chloride etc |