5CF1
Crystal Structure of the M32V/M78V/I80V/L114F mutant of LEH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.783, 60.989, 119.287 |
| Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
| Resolution | 23.911 - 2.242 |
| R-factor | 0.1908 |
| Rwork | 0.188 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nu3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.122 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.242 | 2.250 |
| Number of reflections | 69965 | |
| <I/σ(I)> | 34.1 | |
| Completeness [%] | 97.3 | 94.3 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 1.4M Na-citrate, 0.1M hepes |
