5CER
Bd0816 Predatory Endopeptidase from Bdellovibrio bacteriovorus in complex with immunity protein Bd3460
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92001 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 237.450, 212.320, 90.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.480 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cec |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.683 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 2.480 | |
| Number of reflections | 153169 | |
| <I/σ(I)> | 15.1 | 15.1 |
| Completeness [%] | 99.0 | |
| Redundancy | 13.5 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M Na HEPES pH 7.5, 0.2M MgCl2, 25% w/v PEG 3350 |
