5CEM

Pseudokinase and C-terminal extension of Human Tribbles Homolog 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-02
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.954
Spacegroup name	P 63
Unit cell lengths	81.384, 81.384, 85.883
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.900 - 2.100
R-factor	0.17862
Rwork	0.176
R-free	0.22299
RMSD bond length	0.010
RMSD bond angle	1.385
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.900	2.210
High resolution limit [Å]	2.100	2.100
Number of reflections	18911
<I/σ(I)>	21.3	3.2
Completeness [%]	100.0	100
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	1.0 M Ammonium sulfate, 0.1 M Bis-Tris (pH 5.5), 1% w/v Polyethylene glycol 3,350