5CEL
CBH1 (E212Q) CELLOTETRAOSE COMPLEX
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1995-09-02 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 83.050, 82.950, 110.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.500 - 1.900 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24000 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | CCP4 |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.900 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.067 | 0.402 |
Number of reflections | 29299 | |
<I/σ(I)> | 5 | 1.5 |
Completeness [%] | 96.4 | 92.7 |
Redundancy | 4.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | MES | 100 (mM) | |
3 | 1 | reservoir | mPEG5000 | 20 (%(w/v)) | |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | glycerol | 20 (%(v/v)) | |
6 | 1 | reservoir | cellotetraose | 5 (mM) |