5CEK

Pseudokinase domain of Human Tribbles Homolog 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-02
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.954
Spacegroup name	P 62 2 2
Unit cell lengths	152.693, 152.693, 62.798
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.500 - 2.800
R-factor	0.20937
Rwork	0.207
R-free	0.24554
RMSD bond length	0.008
RMSD bond angle	1.376
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.097
Number of reflections	11093
<I/σ(I)>	18.6	2
Completeness [%]	100.0	100
Redundancy	9.6	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	290	0.1 M Bis-Tris, 2-3 M NaCl, 0.1 M Sodium Citrate and 0.01 M reduced L-Glutathione, 0.01 M oxidized L-Glutathione