5CEK
Pseudokinase domain of Human Tribbles Homolog 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-02 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.954 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 152.693, 152.693, 62.798 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.500 - 2.800 |
R-factor | 0.20937 |
Rwork | 0.207 |
R-free | 0.24554 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.376 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.097 | |
Number of reflections | 11093 | |
<I/σ(I)> | 18.6 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.6 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 0.1 M Bis-Tris, 2-3 M NaCl, 0.1 M Sodium Citrate and 0.01 M reduced L-Glutathione, 0.01 M oxidized L-Glutathione |