5CEE
Malic enzyme from Candidatus Phytoplasma AYWB in complex with NAD and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-07-17 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 48.236, 48.236, 291.068 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.256 - 2.498 |
R-factor | 0.1568 |
Rwork | 0.146 |
R-free | 0.19470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dvm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.151 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.470 | 2.630 |
High resolution limit [Å] | 2.498 | 2.498 |
Rmerge | 0.066 | 0.701 |
Number of reflections | 14448 | |
<I/σ(I)> | 20.2 | 2.4 |
Completeness [%] | 98.4 | 90.7 |
Redundancy | 6.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M MgCl2; 0.1 M Tris pH 8.5; 30% PEG 4000 |