5CED
Penicillin G Acylated Bd3459 Predatory Endopeptidase from Bdellovibrio bacteriovorus in complex with immunity protein Bd3460
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.540, 59.160, 192.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.020 - 2.020 |
| R-factor | 0.1733 |
| Rwork | 0.172 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cec |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.020 |
| Number of reflections | 37468 |
| <I/σ(I)> | 16.2 |
| Completeness [%] | 99.9 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.1M citrate pH 5.0, 3.2M ammonium sulphate |
