5CEB
Bd3459 Predatory Endopeptidase from Bdellovibrio bacteriovorus, K38M form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91739 |
Spacegroup name | P 1 |
Unit cell lengths | 55.930, 65.040, 73.816 |
Unit cell angles | 63.89, 83.27, 83.20 |
Refinement procedure
Resolution | 66.110 - 1.930 |
R-factor | 0.1976 |
Rwork | 0.196 |
R-free | 0.23220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v39 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.629 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 1.930 |
Number of reflections | 56655 |
<I/σ(I)> | 16.2 |
Completeness [%] | 85.7 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M potassium thiocyanate, 30% w/v PEG 2000 MME |