5CE9
structure of tyrosinase from walnut (Juglans regia)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.430, 90.940, 86.580 |
| Unit cell angles | 90.00, 130.18, 90.00 |
Refinement procedure
| Resolution | 44.098 - 1.800 |
| R-factor | 0.1706 |
| Rwork | 0.170 |
| R-free | 0.18750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p3x |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.989 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | |
| High resolution limit [Å] | 1.800 | |
| Rmerge | 0.098 | 0.980 |
| Number of reflections | 88706 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 5000 MME, ammonium sulfate, MES |
