5CDK
Apical domain of chloroplast chaperonin 60b1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-12-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.866, 90.757, 31.551 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.551 - 1.500 |
R-factor | 0.1407 |
Rwork | 0.139 |
R-free | 0.16590 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.622 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.560 |
High resolution limit [Å] | 1.490 |
Number of reflections | 30082 |
<I/σ(I)> | 37.8 |
Completeness [%] | 99.9 |
Redundancy | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 0.05 M Calcium chloride dehydrate, 0.1 M BIS-TRIS pH 6.5, 30% v/v Polyethylene glycol monomethyl ether 550 |