5CDJ
apical domain of chloroplast chaperonin 60a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-06-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 33.912, 53.024, 59.311 |
Unit cell angles | 110.02, 106.32, 91.42 |
Refinement procedure
Resolution | 32.330 - 1.750 |
R-factor | 0.1869 |
Rwork | 0.186 |
R-free | 0.21060 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.781 |
Data reduction software | CNS |
Data scaling software | CNS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.330 |
High resolution limit [Å] | 1.750 |
Number of reflections | 35856 |
<I/σ(I)> | 28 |
Completeness [%] | 96.3 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1 M Tris pH 8.5, 2.0 M ammonium sulfate |