5CDI
Chloroplast chaperonin 60b1 of Chlamydomonas
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-07-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 143.338, 174.390, 213.678 |
Unit cell angles | 90.00, 94.69, 90.00 |
Refinement procedure
Resolution | 48.657 - 3.807 |
R-factor | 0.276 |
Rwork | 0.275 |
R-free | 0.32540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xck |
RMSD bond length | 0.002 |
RMSD bond angle | 0.597 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.660 | 3.870 |
High resolution limit [Å] | 3.807 | 3.800 |
Rmerge | 0.970 | |
Number of reflections | 101331 | |
<I/σ(I)> | 18.27 | 18.27 |
Completeness [%] | 96.7 | 93.6 |
Redundancy | 4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 10% w/v Polyethylene glycol monomethyl ether 5000, 5% v/v Tacsimate pH 7.0, 0.1 M HEPES pH 7.0 |